The Hybridization Probe format is used for DNA detection and
quantitation, providing maximum specificity for product identification.
Two specifically designed, sequence-specific oligonucleotide
probes, labeled with different dyes, are used. The sequences
of the probes are selected so that they can hybridize to the
target sequences on the amplified DNA fragment in a head-to-tail
orientation, thus bringing the two dyes into close proximity.
The donor dye (fluorescein) is excited by the blue light source
and emits green fluorescent light at a slightly longer wavelength.
At close proximity, the energy emitted excites the acceptor dye
attached to the second Hybridization Probe, which then emits
fluorescent light at a different wavelength. The amount of fluorescence
emitted is directly proportional to the amount of target DNA
generated during the PCR reaction. This page provides a link
to Hybridization Probe sets that have been synthesized, tested
and optimized for the measurement of gene expression in the organisms