Hybridization Probes

The Hybridization Probe format is used for DNA detection and quantitation, providing maximum specificity for product identification. Two specifically designed, sequence-specific oligonucleotide probes, labeled with different dyes, are used. The sequences of the probes are selected so that they can hybridize to the target sequences on the amplified DNA fragment in a head-to-tail orientation, thus bringing the two dyes into close proximity. The donor dye (fluorescein) is excited by the blue light source and emits green fluorescent light at a slightly longer wavelength. At close proximity, the energy emitted excites the acceptor dye attached to the second Hybridization Probe, which then emits fluorescent light at a different wavelength. The amount of fluorescence emitted is directly proportional to the amount of target DNA generated during the PCR reaction. This page provides a link to Hybridization Probe sets that have been synthesized, tested and optimized for the measurement of gene expression in the organisms listed below.