Hydrolysis Probes

The Hydrolysis Probe or Taqman chemistry relies on the 5'-3' exonuclease activity of Taq polymerase, which degrades a hybridized non-extendible DNA probe during the extension step of the PCR. This probe is designed to hybridize to a region within the amplicon and is duel labeled with a reporter dye and a quenching dye. The close proximity of the quencher suppresses the fluorescence of the reporter dye. Once the exonuclease activity of Taq polymerase degrades the probe, the fluorescence of the reporter increases at a rate that is proportional to the amount of template present. This page provides a link to Hydrolysis Probe sets that have been synthesized, tested and optimized for the measurement of gene expression in the organisms listed below.