The Hydrolysis Probe or Taqman chemistry relies on the 5'-3'
exonuclease activity of Taq polymerase, which degrades a hybridized
non-extendible DNA probe during the extension step of the PCR.
This probe is designed to hybridize to a region within the amplicon
and is duel labeled with a reporter dye and a quenching dye.
The close proximity of the quencher suppresses the fluorescence
of the reporter dye. Once the exonuclease activity of Taq polymerase
degrades the probe, the fluorescence of the reporter increases
at a rate that is proportional to the amount of template present.
This page provides a link to Hydrolysis Probe sets that have
been synthesized, tested and optimized for the measurement of
gene expression in the organisms listed below.