Forward Primer:5'- GCACCATCCTCCTCTTCCT -3'

Reverse Primer: 5'- GGCCTCTGATAACCAAGCC - 3'

Probe Sequence: 5'- TM2-CCTCCTGAGCACCCTCCTCCTCTCC -3'

Product Size: 426 b.p.

Primer Concentration: 0.8 micromolar

Probe Concentration: 0.05 micromolar

Annealing Temperature: 64°C

Magnesium Chloride Concentration: 4 mM

Instrument: Roche LightCycler

Submitted by: Andreas Nitsche

Institution: Medicine and Oncology Dept, Humboldt University, Germany