Primer Programs
Primer3 (Whitehead Institute)
Raw
Primer (University of Minnesota)
Web Primer (Stanford University)
Primer/Probe Manufacturers
Idaho
Technologies, Inc.
Synthegen,
LLC
Operon Technologies, Inc.
Genset
Corp.
Integrated
DNA Technologies
Gene Express
National Enterprises
TIB
Molbiol
Tamar Ltd.
Instruments
Roche
LightCycler
Perkin Elmer ABI Prism 7900HT Sequence Detection System
Perkin Elmer ABI Prism 7700 Sequence Detection System
Perkin Elmer GeneAmp 5700 Sequence Detection System
BioRad
iCycler
Other Resources for PCR and
Molecular Biology
The Bio-Web - Resources
for molecular and cell biologists
Real
time PCR - The Web page for information and links on RT PCR
Molecular Biology
Gateway - The gateway to Web resources for molecular biology,
genomics, PCR, protocols, microbiology, and biochemistry.
The Molecular Biology Directory
- The independent guide to molecular biology. Lists all major
molecular biology pages and resources.
Real-Time
PCR: An Essential Guide - Real-time PCR Book
Biocompare.com -
Selection of Real time PCR Instruments & Reagents
PCR Primer
Design - Learn how to optimally design PCR primers and reaction
conditions
Holland, P.M., Abramson, R.D.,
Watson, R. and Gelfand, D.H. (1991) Detection of specific polymerase
chain reaction product by utilizing the 5' to 3' exonuclease
activity of Thermus aquaticus DNA polymerase. Proc. Natl.
Acad. Sci. USA 88, 7276-7280.
Tyagi, S. and Kramer, F.R.
(1996) Molecular beacons: prbes that fluoresce upon hybridization.
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Wittwer, C.T., Herrmann, M.G.,
Moss, A.A. and Rasmussen, R.P. (1997) Contiuous fluorescence
monitoring of rapid cycle DNA amplification. Biotechniques
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Rasmussen, R., Morrison, T.,
Herrmann, M. and Wittwer, C. (1998) Quantitative PCR by continuous
fluorescence monitoring of a double strand DNA specific binding
dye. Biochemica 2, 8-11.
Nitsche, A., Steuer, N., Schmidt,
C.A., Landt, O. and Siegert, W. (1999) Different real-time PCR
formats compared for the quantitative detection of human cytomegalovirus
DNA. Clin. Chem. 45, 1932-1937.
Winer, J., Jung, C.K.S., Shackel,
I. and Williams, M. (1999) Development and validation of real-time
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