Primer Programs

Primer3 (Whitehead Institute)

Raw Primer (University of Minnesota)

Web Primer (Stanford University)

Primer/Probe Manufacturers

Idaho Technologies, Inc.

Synthegen, LLC

Operon Technologies, Inc.

Genset Corp.

Integrated DNA Technologies

Gene Express National Enterprises

TIB Molbiol

Tamar Ltd.

Instruments

Roche LightCycler

Perkin Elmer ABI Prism 7900HT Sequence Detection System

Perkin Elmer ABI Prism 7700 Sequence Detection System

Perkin Elmer GeneAmp 5700 Sequence Detection System

BioRad iCycler

Other Resources for PCR and Molecular Biology

The Bio-Web - Resources for molecular and cell biologists

Real time PCR - The Web page for information and links on RT PCR

Molecular Biology Gateway - The gateway to Web resources for molecular biology, genomics, PCR, protocols, microbiology, and biochemistry.

The Molecular Biology Directory - The independent guide to molecular biology. Lists all major molecular biology pages and resources.

Real-Time PCR: An Essential Guide - Real-time PCR Book

Biocompare.com - Selection of Real time PCR Instruments & Reagents

PCR Primer Design - Learn how to optimally design PCR primers and reaction conditions

 

References

Holland, P.M., Abramson, R.D., Watson, R. and Gelfand, D.H. (1991) Detection of specific polymerase chain reaction product by utilizing the 5' to 3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88, 7276-7280.

Tyagi, S. and Kramer, F.R. (1996) Molecular beacons: prbes that fluoresce upon hybridization. Nat. Biotechnol. 16, 49-53.

Wittwer, C.T., Herrmann, M.G., Moss, A.A. and Rasmussen, R.P. (1997) Contiuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 22, 130-138.

Rasmussen, R., Morrison, T., Herrmann, M. and Wittwer, C. (1998) Quantitative PCR by continuous fluorescence monitoring of a double strand DNA specific binding dye. Biochemica 2, 8-11.

Nitsche, A., Steuer, N., Schmidt, C.A., Landt, O. and Siegert, W. (1999) Different real-time PCR formats compared for the quantitative detection of human cytomegalovirus DNA. Clin. Chem. 45, 1932-1937.

Winer, J., Jung, C.K.S., Shackel, I. and Williams, M. (1999) Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. Anal. Biochem. 270, 41-49.