Forward Primer:5'- TGA CCA CCA TTC CCA CCA TA -3'

Reverse Primer: 5'- TTC TGG TTC CGG AGC TGA TT - 3'

Product Size: 415 b.p.

Primer Concentration: 0.2 micromolar

Annealing Temperature: 55°C

Magnesium Chloride Concentration: 4 mM

Instrument: Roche LightCycler

Submitted by: Steven W. Johnson, Ph.D.

Institution: University of Pennsylvania