Forward Primer:5'- ACA AAG AAA CGG CCT TGT CC -3'
Reverse Primer: 5'- TCT CTT GCT GAA TGT GGG CA - 3'
Product Size: 243 b.p.
Primer Concentration: 0.2 micromolar
Annealing Temperature: 55°C
Magnesium Chloride Concentration: 4 mM
Instrument: Roche LightCycler
Submitted by: Steven W. Johnson, Ph.D.
Institution: University of Pennsylvania