Forward Primer:5'- AAA AGA AGC TGG AAG CCG CA -3'

Reverse Primer: 5'- AGG ATT TGA ATT CGG GCG TC - 3'

Product Size: 348 b.p.

Primer Concentration: 1.0 micromolar

Annealing Temperature: 55°C

Magnesium Chloride Concentration: 4 mM

Instrument: Roche LightCycler

Submitted by: Steven W. Johnson, Ph.D.

Institution: University of Pennsylvania