Forward Primer:5'- CTG CGG CAA AAC CTA CAC AA-3'

Reverse Primer: 5'- ATT TTT GGC ACT GGA ACG GG - 3'

Product Size: 176 b.p.

Primer Concentration: 1.0 micromolar

Annealing Temperature: 55°C

Magnesium Chloride Concentration: 4 mM

Instrument: Roche LightCycler

Submitted by: Steven W. Johnson, Ph.D.

Institution: University of Pennsylvania