Forward Primer:5'- TTTGGAGTGAACATCATCTTGAA -3'

Reverse Primer: 5'- ACCAGCTTGTACCCCTGGTAGG - 3'

Product Size: 344 b.p.

Primer Concentration: 0.2 micromolar

Annealing Temperature: 55°C

Magnesium Chloride Concentration: 4 mM

Instrument: Roche LightCycler

Submitted by: Steven W. Johnson, Ph.D.

Institution: University of Pennsylvania