Forward Primer:5'- GAA GTC AGG GGT TTG CGT TA -3'

Reverse Primer: 5'- ATG CCG TCA GTC TGG AGT TT - 3'

Product Size: 294 b.p.

Primer Concentration: 0.2 micromolar

Annealing Temperature: 55°C

Magnesium Chloride Concentration: 4 mM

Instrument: Roche LightCycler

Submitted by: Steven W. Johnson, Ph.D.

Institution: University of Pennsylvania