Secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)

Forward Primer:5'- AGCCAGGACTCCATTGACTCGAAC -3'

Reverse Primer: 5'- GTTTCAGCACTCTGGTCATCCAGC - 3'

Product Size: 416 b.p.

Primer Concentration: 0.2 micromolar

Annealing Temperature: 68°C

Magnesium Chloride Concentration: 4 mM

Instrument: Roche LightCycler/Cephiad SmartCycler

Submitted by: Phoebe S. Leboy

Institution: University of Pennsylvania