Forward Primer:5'- AGG CTT GAC AAA GCT GTT CTG -3'
Reverse Primer: 5'- CCA ACG CTA GAC TGC TCT GA - 3'
Product Size: 500 b.p.
Primer Concentration: 0.2 micromolar
Annealing Temperature: 55°C
Magnesium Chloride Concentration: 4 mM
Instrument: Roche LightCycler
Submitted by: Steven W. Johnson, Ph.D.
Institution: University of Pennsylvania