Forward Primer:5'- TTCTGGTCCTCAGCATCTTC -3'

Reverse Primer: 5'- ACACAAGTCTCAACCCTTCC - 3'

Product Size: 549 b.p.

Primer Concentration: 0.4 micromolar

Annealing Temperature: 56°C

Magnesium Chloride Concentration: 4 mM

Instrument: Roche LightCycler

Submitted by: Dr. Steven Albelda

Institution: University of Pennsylvania