Forward Primer:5'- TTTCTCAGTCCCTCCATTCC -3'

Reverse Primer: 5'- GACTCCATTCCCAAGTTTCC - 3'

Product Size: 689 b.p.

Primer Concentration: 0.5 micromolar

Annealing Temperature: 53°C

Magnesium Chloride Concentration: 4 mM

Instrument: Roche LightCycler

Submitted by: Dr. Steven Albelda

Institution: University of Pennsylvania