Forward Primer:5'- TGTTTGAGACCTTCAACACC -3'

Reverse Primer: 5'- TAGGAGCCAGAGCAGTAATC - 3'

Product Size: 600 b.p.

Primer Concentration: 0.25 micromolar

Annealing Temperature: 57°C

Magnesium Chloride Concentration: 4 mM

Instrument: Roche LightCycler

Submitted by: Dr. Steven Albelda

Institution: University of Pennsylvania