Forward Primer:5'- AGGTAGAGACAGGAGGGTTGC - 3'

Reverse Primer: 5'- TGAAACAGTTCTTTCGTTTTTCTG - 3'

Product Size: 244 b.p.

Primer Concentration: 10 pM

Annealing Temperature: 58°C

Magnesium Chloride Concentration:

Instrument: Roche LightCycler

Submitted by: Dr. Vasudeva Ginjala

Institution: Dept. of Genetics, University of Pennsylvania