SYBR Green I dye intercalates into double-stranded DNA and produces a fluorescent signal. The intensity of the signal is proportional to the amount of dsDNA present in the reaction. Therefore, at each step of the PCR reaction, the signal intensity increases as the amount of product increases. This provides a very simple and reliable method to monitor PCR reactions in real time. Another advantage of this technique is that unmodified oligonucleotide primers are required which facilitates primer design/synthesis and lowers cost. However, optimization of the reaction conditions for each primer set are required. These parameters include primer concentration, annealing temperature and magnesium chloride concentration. This page provides a link to primer sets that have been synthesized, tested and optimized for the measurement of gene expression in the organisms listed below.