SYBR Green I dye intercalates into double-stranded DNA and
produces a fluorescent signal. The intensity of the signal is
proportional to the amount of dsDNA present in the reaction.
Therefore, at each step of the PCR reaction, the signal intensity
increases as the amount of product increases. This provides a
very simple and reliable method to monitor PCR reactions in real
time. Another advantage of this technique is that unmodified
oligonucleotide primers are required which facilitates primer
design/synthesis and lowers cost. However, optimization of the
reaction conditions for each primer set are required. These parameters
include primer concentration, annealing temperature and magnesium
chloride concentration. This page provides a link to primer sets
that have been synthesized, tested and optimized for the measurement
of gene expression in the organisms listed below.